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KMID : 1159320180200040531
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Korean Journal of Vision Science
2018 Volume.20 No. 4 p.531 ~ p.541
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Acute Cytotoxicity Testing of Polyhexamethylene-biguanide (PHMG) and Epigallocatechin-gallate (EGCG) Mixture on the Cultured Human Corneal Epithelial Cell
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Kim Nam-Youl
Lee Koon-Ja
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Abstract
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Purpose : To evaluate the acute cytotoxic effect of polyhexamethylene biguanide (PHMB), epigallocatechin gallate (EGCG) and PHMB/EGCG mixture on the cultured human corneal epithelial cell (HCEpiC).
Methods : HCEpiCs were cultured in the media of HCEpiC containing 0.00001~0.005% PHMB, 0.001~5% EGCG and 0.00005% PHMB/0.05% EGCG mixture respectively for 30, 60, 120 and 240 min. Cultured HCEpiCs were fixed and stained with Draq5 and cell viability and apoptosis were evaluated using confocal microscope and ImageXpress UltraTM.
Results : Cultured HCEpiC did not show cytotoxic effect at below 0.00005% PHMB and below 0.05% EGCG concentration. In the media containing 0.00005% PHMB/0.05% EGCG, acute cytototoxic effect was not found, whereas damaged HCEpiCS were increased and survival cells were decreased in the media incubated for 240 min.
Conclusion : The mixture of 0.00005% PHMB/0.05% EGCG showed non acute cytotoxic effect on the cultured HCEpiCs, however it is needed to investigate its chronic cytotoxic effect.
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KEYWORD
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Cell toxicity, EGCG, Human Corneal epithelial cells, PHMB
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